Notebook – Protocol – 7. Ligation

1. Ligation reaction was as follow:

• Insert DNA, 2,5 ul*
• Plasmid DNA, 1.5 ul*
• Ligation kit (Takara), 5 ul
Total volume, 10 ul
* final quantity insert DNA > plasmid DNA

2. Samples were incubated at 4 oC for overnight (inside a refrigerator).

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