From 2014.igem.org

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30^th June

• Test to synthesize gene SLP with oligos
  Preparation of enzyme mix in ice
  o 80 μl ISO Buffer 5X
  o 5 μl phusion polymerase
  o 40 μl Taq ligase
  o Qsp 300 μl dH2O
  
  Gibson protocol
  o 1 μl dNTP
  o 3 μl oligo at 10ng/μl
  o Qsp 15 μl dH2O
  --> Put 2h at 50°C
  

• Purification of ELP-20, -40 and -60