From 2014.igem.org

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27^th June

• IPTG
  Induction with 8ml IPTG
  m=0,19g qsp 8ml H2O for 1L
  
  
• Oligos SLP
  We try to synthesize SLP gene with oligos
  
  Test with nanodrop :
  [SLP1]=1036,6ng/µl
  [SLP2]=1311,6ng/µl
  [SLP3]=1430,3ng/µl
  [SLP4]=1421,2ng/µl
  [SLP5]=1494,5ng/µl
  [SLP6]=1279,7ng/µl
  [SLP7]=1515,8ng/µl
  [SLP8]=541,4ng/µl
  
  
• Protocol :
  - Oligo (10ng)
  - dNTP(1µl)
  - mix enzyme (15µl)
  
  We want a SLP concentration of 10ng/µl
  We do a mix and put it at 50°C
  

• ELP-20, -40 and -60 in LB+glucose
  Preculture inoculate the previous day

• SDS-PAGE
  10µl P2 + 10µl sample 2X -> 5min at 85°C
  6µl ladder