Team:Wageningen UR/notebook/journal/kill-switch

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Wageningen UR iGEM 2014

 

 

Journal


Overview

Primers used to make promoters:

  • Tet Tet fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACAACGTCGTGTTAGCTGCCTTTCGTCTTCAATAATTCTTGACAAATAACTCTA
  • Tet Tet fin primer
    RVS:GGCCGCTACTAGTAGTTGGGTAACGCTCTCTATCACTGATAGGGGTGGAACTCTATCATTGATAGAGTTATTTGTCAAGAATTAT
  • Tet – Tet fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACAACGTCGTGTTAGCTGCTCCCTATCAGTGATAGAGATTGACATTTATGCTTC
  • Tet – Tet fin primer
    RVS:GGCCGCTACTAGTAGTTGGGTAACGCTCTCTATCACTGATAGGGGTGGAATTATACGAGCCGGAAGCATAAATGTCAATCTCTAT
  • CI Lac Lac fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTAGACATTGTGAGCGG
  • CI Lac Lac fin Primer
    RVS:GGCCGCTACTAGTAGTTGGTTGTTACTCGCTCACATTTAAATTGCACGAAGTATCTTGTTATCCGCTCACAATGTCTAACACCGT
  • Lac CI Lac fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACAACGTCGTGTTAGCTGCAATTGTGAGCGGATAACAATTGACTATTTTACCTC
  • Lac CI Lac fin primer
    RVS:GGCCGCTACTAGTAGTTGGTTGTTACTCGCTCACATTTAAATTGCACGAATTATCACCGCCAGAGGTAAAATAGTCAATTGTTAT
  • CI Tet Tet fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTAGACAAATAACTCTA
  • CI Tet Tet fin primer
    RVS:GGCCGCTACTAGTAGTTGGGTAACGCTCTCTATCACTGATAGGGGTGGAACTCTATCATTGATAGAGTTATTTGTCTAACACCGT
  • Tet CI Tet fin primer
    FW:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTACAACGTCGTGTTAGCTGCTCCCTATCAGTGATAGAGATTGACTATTTTACCTC
  • Tet CI Tet fin primer
    RVS:GGCCGCTACTAGTAGTTGGGTAACGCTCTCTATCACTGATAGGGGTGGAATTATCACCGCCAGAGGTAAAATAGTCAATCTCTAT

Protocols used:

Protocols made:


July

Week 1-4

August

Week 1-4

September

Week 1-4

October

Week 1-2



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