"
Team:Arizona State/policypractices
From 2014.igem.org
| |||||||||||
 |
|||||||||||
| | |||||||||||
|
|
Policy
Policy and Practices text
Policy and Practices text
Practices
Resting Cell Assay To test the productivity of different plasmids, resting cell assays were run. All assays were run in a shaker incubator at 32 degrees C and 200 RPM. A phosphate buffered solution was used to suspend the growth of the cultures.
Quantification A GC with a DB5 column was used to quantify data from all assays. For testing ethanol production, the method used had an initial temp. of 60 degrees C, and ramped by 10 degrees C until it reached 250 degrees C, which was then held for 3 minutes. |
||||||||||
 | |||||||||||
