Team:Freiburg/Content/Notebook
From 2014.igem.org
p14rz_002
- PCR: o14rz_005, o14_rz006; Template pQXCIN-SLC7A1
- Digest: pKM006, EcoRI + NotI; PCR-product
- Testdigest with PstI in Cutsmart, expected bands: 5,9; 0,7
![](https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/d/dc/2014Freiburg_140729Testverdau_006%2BSLC%2BHA_PstI_006%2BSLC_PstI.jpg)
Testdigest
p14rz_003
- PCR 3
- Digest 3, 4
- Testdigest with PstI + SpeI in Cutsmart, expected bands: 4,1;2,1;1,8
![](https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)
Testdigest
p14rz_004
- PCR 21,22
- Digest -
- Testdigest with PstI in Cutsmart, expected bands: 4,1;3,2
![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg)
Testdigest
p14rz_005
- PCR 4,5
- Digest 5
- Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,3
![](https://static.igem.org/mediawiki/2014/5/5f/2014Freiburg_140811_PCR1%2C2%2C4%2C5%2C6%2C7%2C9.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)
Digest
p14rz_006
- PCR 17,18
- Digest 5
- Testdigest with AgeI+HindIII in Cutsmart, expected bands: 6,6;1,4
![](https://static.igem.org/mediawiki/2014/5/5e/2014Freiburg_140811_PCR1%2C17%2C18.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/b/bc/2014Freiburg_140813_Testverdau_rz005_rz_006_je_8x_ls003_3x_Retrafos.jpg)
Digest
Testdigest
p14rz_007
- PCR 37,38
- Digest 21
- Testdigest with PstI in Cutsmart, expected bands: 4,1;2,2;1,7
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/0/00/2014Freiburg_140923_Testverdau_Gibson_16_1x_Gibson_17%2C31%2C33%2C34_je_3x_a.jpg)
Testdigest
p14rz_008
- PCR blue light receptor; Template: pQXCIN-SLC7A1
- Digest pKM006 EcoRI, NotI
- Testdigest with SmaI in Cutsmart, expected bands: 5,7
![](https://static.igem.org/mediawiki/2014/0/07/2014Freiburg_140726_Verdaue_Rezeptor_normal%2CHA_pKM006%2C_PCR_LoveIRES_Rezeptor_Blaulicht.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/c/c8/2014Freiburg_140729Testverdau_084%2BSLC_SmaI.jpg)
Testdigest
Testdigest
p14rz_009
- PCR 39,40
- Digest 5
- Testdigest with BamHI in Cutsmart, expected bands: 3,0;2,7
PCR
![](https://static.igem.org/mediawiki/2014/f/fa/2014Freiburg_140811_Verdau_pLXV_MluI%2BClaI_pcDNA_BamHI%2BNotI_p14vv003_XbaI.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)
Testdigest
p14rz_010
- PCR 23,24
- Digest 12
- Testdigest with PstI in Cutsmart, expected bands:3,1;2,3;0,56
![](https://static.igem.org/mediawiki/2014/9/93/2014Freiburg_140820_PCR_22%2C23%2C9%2Cdigest12.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/c/c0/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_2_b.jpg)
Testdigest
p14ls_002
- PCR 7,8
- Digest -
- Testdigest with PstI in Cutsmart, expected bands: 4,1;0,56
PCR
Digest
Testdigest
p14ls_003
- PCR 7,9,10
- Digest -
- Testdigest with KpnI in Cutsmart, expected bands: 4,9; 1,3; 0,4
![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/60/2014Freiburg_140820_PCR_7_2x_PCR_28_2x.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/0/05/2014Freiburg_140822_Testverdaue_ls_003_5x_rz004_5x_rz010_5x_vv006_5x_alle_positiv_a.jpg)
Testdigest
p14vv_001
- PCR Primers vv003,vv004; Template: pMIG
- Digest pMIG EcoRI,NotI; C1-eGFP EcoRI,NotI
- Testdigest with NgoMIV in Cutsmart, expected bands: 5,7; 0,6
![](https://static.igem.org/mediawiki/2014/b/b7/2014Freiburg_140719_PCR_fuer_Blaulicht_puro_pKM297_pKM022_pKM292_erfolgreich_55_60_Grad_c1_egfp_plxv_verdau.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140728_Testverdau_pMIG_CMV_1-4_pMIG_MCS_unverdaut2x_verdaut_6x.jpg)
Testdigest
p14vv_002
- PCR -
- Digest 8,11
- Testdigest with PstI in Cutsmart, expected bands: 3,2;2,1;1,4
![](https://static.igem.org/mediawiki/2014/0/09/2014Freiburg_140811_PCR10%2C14%2C15%2C17%2C18%2C19%2C20.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/1/19/2014Freiburg_140822_Digest8%2C_11%2C_10.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/5/51/2014Freiburg_140825_Testverdau_rz003_KpnI_2x_pSAM_Xba_3x_ls003_PstI_2x_ls001_Bbs_EcoRI_2x_zl005_EcoRV_5x_pMIG%2BSyn_PstI_5x_pMIG-Xba_Xba%2CSpe_5x_b.jpg)
Testdigest
p14vv_003
- PCR pMIG with primer vv003+vv004-
- Digest xx
- Testdigest with KpnI in Cutsmart, expected bands: 3,5;1,5
![](https://static.igem.org/mediawiki/2014/0/06/2014Freiburg_140709_pMIG_vor_Gelelution_3-6b%2CplXV9a-12a.jpg)
PCR
Digest
![](https://static.igem.org/mediawiki/2014/1/1f/2014Freiburg_140713_pMIG_IRES_out_und_pMIG_eGFP_und_IRES_out-Testverdau_.jpg)
Testdigest
p14vv_006
- PCR 26
- Digest 13
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,5;1,9;0,95,0,6
![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)
PCR
Digest
![](https://static.igem.org/mediawiki/2014/a/a1/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_a.jpg)
Testdigest
p14vv_007
- PCR 27
- Digest 14
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,5; 1,3; 0,97; 0,66
![](https://static.igem.org/mediawiki/2014/c/cd/2014Freiburg_140820_PCR_21%2C25%2C10%2C26%2C27%2C28%2C7%2C24.jpg)
PCR
Digest
![](https://static.igem.org/mediawiki/2014/c/cb/2014Freiburg_9_10_2014_2_13_33_PM_a.jpg)
Testdigest
p14vv_008
- PCR 43
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 1,4 0,75
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)
Testdigest
p14vv_009
- PCR 44
- Digest 22
- Testdigest with EcoRV+MluI in Cutsmart, expected bands: 3,4; 1,43; 0,75
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg)
Testdigest
p14vv_010
- PCR 45
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 3,4; 2,2
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/2/23/2014Freiburg_9_10_2014_2_13_33_PM_b.jpg)
Testdigest
p14vv_012
- PCR 46
- Digest 22
- Testdigest with EcoRV in Cutsmart, expected bands: 4,7;3,4
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)
Testdigest
p14vv_013
- PCR 47,48,49
- Digest 22
- Testdigest with NgoMIV in Cutsmart, expected bands: 3,4;2,0;1,3;0,2
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg)
Testdigest
p14vv_014
- PCR 50,51
- Digest 22
- Testdigest with NgoMIV in Cutsmart, expected bands: 4,1; 1,56; 0,66
![](https://static.igem.org/mediawiki/2014/9/98/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_a.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/6c/2014Freiburg_2014_09_14_Testverdaue_Gibson_10_14_15_16_18_20_23_25_b.jpg)
Testdigest
p14zl_003
- PCR xx
- Digest xx
- Testdigest with NruI+MluI in Cutsmart, expected bands: 4,9;3,3;2,7;0,7
- Testdigest with NgoMIV in Cutsmart, expected bands: 4,7;4,1;2,8
PCR
Digest
Testdigest
p14zl_010
- PCR 1,2
- Digest 1
- Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,4;1,1
![](https://static.igem.org/mediawiki/2014/8/81/2014Freiburg_140814_PCR_1%2C2%2C3%2C12%2C13.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)
Testdigest
p14zl_011
- PCR 19,20
- Digest 1
- Testdigest with NgOMIV in Cutsmart, expected bands: 4,7;2,0;1,3;0,43
![](https://static.igem.org/mediawiki/2014/b/b0/2014Freiburg_140814_PCR_16%2C19%2C20_Verdau_pLXV_MluI_ClaI_Verdau_pcDNA_BamHI_HindIII.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/6/62/2014Freiburg_140819_Testverdaue_rz_003_5x_pMIG_Xba_raus_4x_ls001_5x_zl010_zl011_je_5x_b.jpg)
Testdigest
p14zl_016
- PCR 52,53
- Digest 22
- Testdigest with HindIII in Cutsmart, expected bands: 5,7;2,3;1,7
![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6a/2014Freiburg_140716_pMIG-verdaut_mit_EcoRI_und_NotI_zum_Einf%C3%BCgen_des_CMV-eGFP.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/0/0f/2014Freiburg_2014_09_14_Testverdaue_Gibson_24_26_28_29_a.jpg)
Testdigest
p14zl_017
- PCR 64,65
- Digest 24
- testdigest with NgoMIV in Cutsmart, expected bands: 5,4;2,9
![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)
PCR_digest
PCR
![](https://static.igem.org/mediawiki/2014/b/b3/2014Freiburg_2014_09_14_Testverdaue_Gibson_32_31.jpg)
Testdigest
p14zl_018
- PCR 55,56
- Digest 24
- testdigest with NgoMIV in Cutsmart, expected bands: 4,7;2,5
PCR
Digest
Testdigest
p14zl_019
- PCR 57,58
- Digest 24
- testdigest with PstI in Cutsmart, expected bands: 5,8;2,6;1,1;0,2
PCR
Digest
Testdigest
p14zl_020
- PCR 59,60
- Digest 24
- testdigest with BamHI-HF, NgoMIVin Cutsmart, expected bands: 4,7; 2,4; 1,8
![](https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg)
PCR
![](https://static.igem.org/mediawiki/2014/6/6d/2014Freiburg_2014_10_16_PCR37%2C40%2C43%2C44%2C45%2C46%2C47%2C48%2C49%2C50%2C51%2C52%2C53%2C59%2C60%2C64%2Cdigest23%2Cdigest24%2Cdigest23_b.jpg)
Digest
![](https://static.igem.org/mediawiki/2014/5/52/2014Freiburg_9_10_2014_2_10_51_PM.jpg)
Testdigest
p14zl_023
- PCR 68,69
- Digest 22
- testdigest with EcoRV in Cutsmart, expected bands: 3,4;2,5
PCR
Digest
Testdigest