From 2014.igem.org
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Notebook |
7/14/14 - Project began. Started planning project timeline and methods/materials needed to complete project.
7/16/14 - Ordered primers for CysK-T25 OE PCR project, 4 primers total:
Primer #1: CysK-EcoRI Forward-tttgaattcATGAGTAAGATTTTTGAAGATAACTCG
Primer #2: CysK-OE reverse - cccgcttgctccagtcccCTGTTGCAATTCTTTCTCAGTG
Primer #3: T25-PstI reverse - tttctgcagaTTATATCGATGGTGCAGCC
Primer #4: T25-OE Forward - gggactggagcaagcgggCAGCAATCGCATCAGGC
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