Team:Goettingen/notebook materials

Materials

Antibiotics
Ampicillin 100 µg/mL
Gentamicin 10 µg/mL
Kanamycin

Supplements
Tryptophane 50 mg/l
Histidine 20 mg/L
Leucine 100 mg/L
Uracile 20 mg/L
Adenine 100 mg/L
3-50 mM 3-amino-1,2,4-triazole (3-AT)

LB medium
10 g Tryptone
5 g Yeast Extract
10 g NaCl
add optional:
15 g agar-agar

50 x TAE buffer
242 g Tris
57,1 mL Acetid acid (100%)
100 mL EDTA (0,5 M; pH 8.0)
Dissolve first IN 60% of water, add acid ad. 1 L

YPD medium
20 g Tryptone
10 g Yeast Extract
20 g Glucose
add optional:
15 g agar-agar

YPAD medium
20 g Tryptone
10 g Yeast Extract
100 mg Adenine
Adjust pH to 5.8
after autoclaving:
50 mL Glucose (40%)
add optional:
15 g agar-agar

SC dropout medium
6.7 g Yeast Nitrogen Base (without amino acids)
1,46 g Amino acid mixture (-Leu/-Trp/-His/-Ura)
Adjust pH to 5.6
Autoclave 15 min only at 121°C
Add 50 mL sterile Glucose (40%), possible to add 3-50 mL 3-AT

SOB medium
20 g tryptone
5 g Yeast extract
0,584 g NaCl
0,186 g KCl
ad dH2O to 1000 mL
2,032 g MgCl2
2,064 g MgSO4
pH with NaOH to 7.0