Team:Penn/Protocol

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University of Pennsylvania iGEM

Protocols

Miniprep Cloning PCR Colony PCR Gel Extraction PCR Cleanup Restriction Enzyme Digest Ligation Transformation Inoculation E.Coli Specific Protocols

Gel Extraction

AMB-1 Specific Protocols

Our protocols for working with AMB-1 have been included in this spec sheet. However, for easy access to consolidated, optimized protocols, refer to the AMB-1 Specification Sheet we developed.

Glycerol Stock

MSGM Media Preparation

EMSGM Media Preparation

Inoculation of Aerobic Cultures

Counting Cell Cultures

Transformation Protocol 1

Transformation Protocol 2

Penn iGEM Transformation Protocol

Plating Techniques