Team:Oxford/Results

Calculating the pH change

We then used our model to predict the effect on the system if you simply increase the amount of water in the aqueous layer. This shows how much water is necessary to prevent the pH from dropping too much. It demonstrates why addition of a buffer is the more reasonable choice to control the pH of the system.

The graph here is for non specific inputs and is for demonstration purposes only. It shows well how the model responds to changing the input values.

Buffers?

Calculating the pH change

We then used our model to predict the effect on the system if you simply increase the amount of water in the aqueous layer. This shows how much water is necessary to prevent the pH from dropping too much. It demonstrates why addition of a buffer is the more reasonable choice to control the pH of the system.

The graph here is for non specific inputs and is for demonstration purposes only. It shows well how the model responds to changing the input values.

Buffers?

Calculating the pH change Realisation Results:

For the containment of our bacteria, we have managed to:

1. synthesise novel agarose beads that have a polymeric coating which limits DCM diffusion into the beads. This allows optimum degradation by the bioremediation bacteria, while physically containing the bacteria for safety reasons
2. verify the functioning of the biopolymeric beads by measuring diffusion using indigo dye 3. use computer-aided modelling to design a prototype of the DCMation system, and physically constructed this container
4. 3D print a cartridge to hold our biosensor bacteria, which can easily be replaced by the user
5. construct a prototype circuit that lights up when the photodiodes detect light emission from our biosensing bacteria that are contained in the cartridge. This lets the user have a simple yes/no response to whether the contents of the container are safe for disposal.