Protocols

All protocols used by the team are listed on this page and explained in PDF files. We have used standard iGEM protocols and protocols that we derived from literature or designed ourselves.

Custom Protocols

Ammonium Analysis

DAPG Protocols

Fusarium Inhibition

MgL Growth Protocol

Pyoverdine Measurement Protocol

Fusaric Acid inducible promoter growth experiment protocol

Metabolic Modeling Pathways

Promoter Characterisation Protocol

Promoter Assembly

Congo Red Assay

Collaboration protocol from iGEM Delft-Leiden 2014

Standard Protocols

Media & Buffers

Transformations

Used Kits

PCR

Restrictions & Ligations

Mutagenesis