09/29/2014

Today we returned to the laboratory of Mineral Analyzes on CPRM to make our calibration curve that will work as a pattern for mercury quantification on experiments with constructions of the binding protein to mercury (MBP):

Besides that, we also decided to digest each bio brick to be submitted for the competition in a more rigorous way. Starting with BB_Bioremediation, with the following system:

Electrophoretic digestion profile of BB_Bioremediation:

1 : BB_Bioremediation in PSB1C3 not digested

2: BB_Bioremediation in PSB1C3 digested with EcoRI

3: BB_Bioremediation in PSB1C3 digested with PstI

4: BB_Bioremediation in PSB1C3 digested with EcoRI and PstI

5 : BB_Bioremediation in PSB1C3 digested with Xba

6: BB_Bioremediation in PSBK from JM110 digested with Xba

7: BB_Bioremediation in PSBK from JM110 digested with Spe

8: BB_Bioremediation in PSBK from JM110 digested with Xba + Spe.