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From 2014.igem.org

DESIGN OVERVIEW

In order to achieve the goal of integrating the directed evolution processes into a single automatic and controllable system, we come up with the following design.

1.The initiate state

M13 bacteriophage with gⅧ deficient carrying Prey sequence yet to be evolved. An E. coli with pBT-Bait and pRPT-gⅧ. At the temperature of 0 degree Celcius

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2.Infection

When the temperature rise to 30 degree Celcius, the M13 vector infects into the E. coli.

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3.Mutagenesis

When the M13 vector undergo replication, a library of different Prey is generated.

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