Team:SYSU-China/file/Project/Notebook/Labnote/Mutation.html

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Mutation·LABNOTE

May: amplify biobricks

June: clone the WT DNAQ ,recA,umuC,umuD from E.coli

July: perform the point-mutation and truncation of the four genes

August: add prefix and suffix to the genes

&nbsp&nbsp&nbsp&nbspAdd RBS to the genes

September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;

&nbsp&nbsp&nbsp&nbspDNAQ926+umuD’+umuC+recA730;) <p>&nbsp&nbsp&nbsp&nbspAdd promoter to construct the expression vector <p> Test the mutation rate

October: adapt the testing method and test the mutation rates.

&nbsp&nbsp&nbsp&nbspPrepare the biobrick for submission


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