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Team:Northwestern/CFPS
From 2014.igem.org
Cell-Free Protein Synthesis
20140723 Testing our lysates
- data from BL21 (E.Coli) lysates
- out of K12, DH5a, and BL21, BL21 expressed the most fluorescence
- the plasmid used was superfolder GFP from Jewett Lab
- conclusionour lysates are function
20140915 All of our lysates
- RBS B0034
- Tried all of our lysates
- green fluorescence indicates amount of transcription
- red fluorescence indicates amount of translation
- conclusion: the data is most likely statistically meaningless
20141014 Last CFPS attempt with all of our lysates
- We used RBS no. 5 (B0034) with Spinach Aptamer
- Displayed here is the first read
- The kinetic portion of the plate reader did not record data in all of our wells
- We will continue doing CFPS experiments
- conclusion: we need more time! Looks like fluorophores were incorrectly added
