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From 2014.igem.org

17^th June

• Plasmid extraction of VPGXG-20,-40 and VPGXG-60 in pSB6A1 by QIAprep Spin Miniprep Kit from QIAGEN

• A₆₀₀ preculture VPGXG-20 = 1,994
  A₆₀₀ preculture VPGXG-40 = 1,849
  A₆₀₀ preculture VPGXG-60 = 2,177
  
  - Culture of VPGXG-20, VPGXG-40 and VPGXG-60 at 37°C
  
  T=0, A₆₀₀ VPGXG-20 = 0,037
  A₆₀₀ VPGXG-40 = 0,016
  A₆₀₀ VPGXG-60 = 0,018
  
  - IPTG induction when A₆₀₀= 0,8 and culture at 25°C
  
  T=8h, ₆₀₀ VPGXG-20 = 1,845
  A₆₀₀ VPGXG-40 = 1,509
  A₆₀₀ VPGXG-60 = 1,499

• We stop protocol after resuspend cell pellet in 20mL PBS and store at -80°C.

• Pattern : Met-M-[(VPGGV)2VPGQQVPGGY]-Spe1-Stop
• We try to design oligo with this pattern