===[[Team:British_Columbia/Notebook/Protocols/PCR|PCR]]===
Instruction for colony PCR and regular PCR
===[[Team:British_Columbia/Notebook/Protocols/gelverification|Gel Verification]]===
Make a gel and conduct gel elecrophoresis
===[[Team:British_Columbia/Notebook/Protocols/Biobrickdigest|BioBrick Restriction Digest]]===
Restriction digest with BioBrick plasmid an inserts
===[[Team:British_Columbia/Notebook/Protocols/ligation|BioBrick Ligation]]===
Ligate Biobrick insert to vector
===[[Team:British_Columbia/Notebook/Protocols/plates|Making Plates]]===
Make LB plates with antibiotics
===[[Team:British_Columbia/Notebook/Protocols/media|Liquid media]]===
Make liquid LB media
===[[Team:British_Columbia/Notebook/Protocols/compcells|Competent cells]]===
Preparation of chemically competent cells
===[[Team:British_Columbia/Notebook/Protocols/transformation|Transformation]]===
Transformation of plasmi DNA into chemically competent cells
===[[Team:British_Columbia/Notebook/Protocols/RepeatSpacerAssembly |CRISPR Repeat-Spacer Array Assembly]]===
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
===[[Team:British_Columbia/Notebook/Protocols/AnnealedOligonucleotides|Assembling Parts from Oligonucleotides]]===
Assemble short parts from annealed oligonucleotides
===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]===
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]===
Phosphorylate oligonucleotides prior to ligation
===[[Team:British_Columbia/Notebook/Protocols/ElectrocompCells|Electrocompetent Cell Production]]===
Make electrocompetent cells
===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]===
Make combinatorial part libraries from compatible parts
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