Team:UCLA/Notebook/Protocols

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Protocols


Recombinant Silk Proteins using Head to Tail Method Protocol Paper for Producing Recombinant Silk with Head to Tail Method Equipment for Recombinant Silk Production Reagent List for Recombinant Silk Production Outline Construction of the Synthetic Spider Silk Multimeric Gene Cloning of the spider silk multimer into the expression vector Gene Expression of pETsilk E.coli Cell Lysis Silk Protein Purification Using IMAC Dialysis Protein Analysis Amino Acid Analysis Overall: Timing of Procedure

Reference Materials Fabrication Rockwood, Danielle N., Rucsanda C. Preda, Tuna Yücel, Xiaoqin Wang, Michael L. Lovett, and David L. Kaplan. "Materials Fabrication from Bombyx Mori Silk Fibroin." Nature Protocols 6.10 (2011): 1612-631. Web. Production of MASP1/2 Teulé, Florence, Alyssa R. Cooper, William A. Furin, Daniela Bittencourt, Elibio L. Rech, Amanda Brooks, and Randolph V. Lewis. "A Protocol for the Production of Recombinant Spider Silk-like Proteins for Artificial Fiber Spinning." Nature Protocols 4.3 (2009): 341-55. Web.