Parts
We have registered 5 different biobricks in the registry of parts and here you´ll get a small description of every biobrick.
<groupparts>iGEM014 Linkoping_Sweden</groupparts>
BBa_K1447002: Epitope 2 + E1010
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to RFP (E1010).
Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).
Figure 1A. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Frontside of the plate).
Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).
Figure 1B. Agar-plate of transformed bacterial cells with our Biobrick including RFP (E1010) linked to epitope 2. (Backside of the plate).
BBa_K1447005: Epitope 1-5 + J06504
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to MCherry (J06504).
BBa_K1447003: Epitope 2 + J06504
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 2 linked to MCherry (J06504).
Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).
Figure 2A. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Frontside of the plate).
Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2. (Backside of the plate)
Figure 2B. Agar-plate with transformed bacterial cells. One half of the plate includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 1-5 and the other half includes transformed bacterial cells with our Biobrick containing MCherry linked to epitope 2.(Backside of the plate)
BBa_K1447004: Epitope 1-5 + E1010
This biobrick contains a designed sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), a designed His-TEV sequence as well as a designed sequence of epitope 22, epitope 1, epitope 3, epitope 4 and epitope 17 linked to RFP (E1010).
Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).
Figure 3A. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Frontside of the plate).
Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).
Figure 3B. Agar-plate with transformed bacterial cells. All 3 parts of the plate includes different colonies of transformed bacterial cells with our Biobrick containing RFP linked to epitope 1-5. (Backside of the plate).
BBa_K1447007: His-TEV
This biobrick contains the sequence of Promotor T7 + RBS from the biobrick (BBa_K525998), as well as a designed His-TEV sequence.
Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate).
Figure 4A. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Backside of the plate)
Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).
Figure 4B. Agar-plate of transformed bacterial cells with our Biobrick including His-TEV. (Frontside of the plate).
"
