Team:Evry/Notebook/Transposons/15.10.14

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== 14.10.2014 ligation== it gave only one clone. Clone culture: 5 ml LB + 5 ul Kan (25 mg/ml) - 37 C° == Transformation Pseudovibrio denitrificans & Dh5 alpha with mRFP in BBa_1413044== electrocompetent cells: 1 mm cuvette; 1800 V recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha plating: MB agar for Pseudovibrio denitrificans LB agar for LB for DH5alpha == Transformation Pseudovibrio denitrificans & Dh5 alpha with BBa_1413001 in BBa_1413044== electrocompetent cells: 1 mm cuvette; 1800 V recuperation media: MB for Pseudovibrio denitrificans , LB for DH5alpha plating: MB agar for Pseudovibrio denitrificans LB agar for LB for DH5alpha == Verification testing of pnK2 in Pseudovibrio denitrificans

Oct 15

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