Team:Evry/Notebook/Transposons/13.10.14

From 2014.igem.org

Revision as of 19:58, 17 October 2014 by Rbodinier (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Picture

== BBa_1413044 retrieval of the transposon plasmid== Digestion of BBa_1413044 by BglII : 50 ul final volume, 5 ul Buffer NeB 3.1, 5 ul (20 ng/ul) BBa_1413044 in pSB1C3, 1 ul BglII, 39 ul H2O. Gel electrophoresis: GEEEEEEEEEEEEEEEEEEEEEELLLLLLLLLL Purification sur gel: Kit thermoscientific "Gel extraction". Elution final in 30 ul. == Obtaining the Kan gene on pNK2 == PCR: enzyme: Q5; template: 150 ng pNK2; oligo: F 82/ R 83; Tm tested: 55/60/65; elongation time: 1m30s Purification sur gel: Kit thermoscientific "Gel extraction". Elution final in 30 ul.
.

Oct 13

Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transposons/13.10.14"