Team:Wageningen UR/project/low copy

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Wageningen UR iGEM 2014

Looking for low copy number plasmids

Two sets of T-A combinations, Kid-Kis and Zeta-Epsilon which have been studied for biosafety purposes before, were selected for this project [2]. Both are type II T-A systems, meaning that antitoxin protein prevents the toxin protein from functioning by binding.



Figure 1. Mode of action of the double interdependent plasmid system that avoids horizontal gene transfer between BananaGuard (green bacteria) and native bacteria in the soil (yellow bacteria). In case one of the plasmids would be transfer to soil bacteria, it would die due to expression of one of the toxins.


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