Team:Groningen/Template/MODULE/Notebook/detection/week3

Two ligation were performed, the first one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP44 (BBa_K1033225), the second one was the purple-blue chromoprotein amilCP with the double terminator was ligated with promoter CP29 (BBa_K1033222)

Both constructs were transformed to E. coli DH5α

Some blue colonies were visible on the plates, an overnight culture was prepared from these colonies

The plasmids were isolated out of the overnight cultures

The size of the constructs were digested, and checked on gel