10/01/2014

Today we started the experiment for bioaccumulation. The main idea is to let the bacteria let bacteria E. coli DH5α with our construction (BB_Essential + K346004) in liquid medium and after, quantify how much mercury is on nutritive medium and how much it has inside the bacteria. This is due to the bacteria capacity to accumulate mercury inside it

For that, it was tested four different concentrations (0.5 ug/ml; 0.25 ug/ml; 0.1ug/ml; 0.05ug/ml) besides of a sample without mercury. We used as control the same bacteria linage with a gene not related to mercury metabolism (GFP) to analyze the influency of the mercury binding proteins naturally expressed by bacteria.

The procedures were made based on bio detection’s procedures. We also continued the validation and characterization of our parts regarding to genetic-molecular level.