Team:Evry/Interlab Study/09-18-2014

Construction n°1: PSB1C3 with I20260

Construction n°2: PSB1C3 with J23101-E1010

Construction n°3: PSB1C3 with K823012-E1010

Measure of the cells' growth and fluorecence on TECAN infiniteM200 (00h-07h).

Other constructions of the Anderson library of constitutive promoters

PSB1C3+E0240+J23101:

Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h43-23h43).


PSB1C3+E0240+J23102:

Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

Measure of the cells' growth and fluorecence on TECAN infiniteM200 (16h30-23h30).


PSB1C3+E0240+J23104:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23105:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23107:
glycerol stock preparation from LB culture of the 17th September.

PSB1C3+E0240+J23110:
Pre-culture does'nt grow. The TECAN measure is reported.

PSB1C3+E0240+J23115:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23116:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23117:
Pre-culture from glycerol in 3mL of LB+Cam (1:1000). Incubation 16h-18h at 37°C.

PSB1C3+E0240+J23114:

• PCR colony of 5 colonies for each construction following protocol table 1 and 2.
  

  

  GEL 1: 1% agarose gel. PCR product of 5 colonies of the PSB1C3+E0240+J23114 construction.

A pre-culture was made with one colony to make a glycerol stock.

Sep 18