Toggle navigation SCU-Igem Project Background Overview Description Result Modeling Human Practice Presentation at Seventh Senior High School Visiting University of Electronic Science and Technology of China (UESTC) Safety Parts Attributions Team Notebook Notebook Notebook of Biobricks Notebook of Transmitter Notebook of Effector Notebook of Sensor Method Bacterial Genomic DNA Prep Digestion Gel Extraction Linkage PCR Plasmid Mini Prep Digestion Protocol Back to top According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20μl). DNA 250ng Enzyme 1 1μl Enzyme 2 1μl 10 X Buffer 2μl H2O Up to 20μl Sichuan university
Digestion Protocol
According to the demand of IGEM Competition, we usually use the EcoR I, Xba I, Spe I, and Pst I to do the digestion and the following is our system (20μl).
DNA
250ng
Enzyme 1
1μl
Enzyme 2
10 X Buffer
2μl
H2O
Up to 20μl
Sichuan university
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