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Team:Penn State/CodonOptimization
From 2014.igem.org
WELCOME TO PENN STATE iGEM 2014!(Page under construction) |
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CODON OPTIMIZATION PROJECT | |||||||||||||
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Codon Optimization: Engineering a More Useful Gene at the Codon Level
Project Summary
Codons are groups of three nucleotides that specify a single amino acid, which is then added to a growing polypeptide chain during translation. Even though each codon spefifies only one amino acid, some amino acids are coded by multiple codons. It has been demonstrated that the genome of E.coli shows statistical preference for some of these degenerate codons over others, and it is hypothesized that these codons translate more efficiently than non preferred degenerate codons. We constructed synthetic reporter genes entirely from codons hypothesized to be fast or slow,and characterized them in E.coli, demonstrating that...
Why is this important?
Background
Our Objectives
1) Objective 1
2) Objective 2
3) Objective 3
4) Objective 4
5) Objective 5
Design Methods
The figure below shows the vector pFTV that was altered using inverse PCR.
Inverse PCR
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