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Team:Warsaw/EXTRAS
From 2014.igem.org
Technology
Bioprocess
Electronic equipment is the fastest growing waste category of waste in many developed countries. Amount of electronic waste grows rapidly because markets in which electronic is produced cross the other side of the ‘Digital Divide’. We stand in front of the following problem: what we are we going to do with all those computers, smartphones which we buy? All these products become obsolete or just unwanted within 1-3 years of purchase. Where will we find a new source of metals necessary to fabricate electronic equipment? Using old and broken WEEE in our project not only gives us a source of metals to produce new smartphones or computers, but also solves problem of storing WEEE.
This figure shows a view on the context of re-use of EEE or its components. At the end of the use EEE returns to producers or to companies, which specialize in reuse of e-waste. The next step is preparation for re-use. On this step wasted electronic equipment is deconstructed. Not only computer memories or other parts which contains valuable metals are re-use. We also want to recover such materials as plastic or glass. That step requires labor work or special machines which will prepare WEE for another point of the plan. Some parts of the TVs or computers may not be useful in another manufactory. Sometimes we need to remarked parts which we need and work in place with special equipment. In the future we can use our bacteria with lanthanides binding sequences to re-use lanthanides. 1) Removing from WEEE plastic and stirring parts which are abundant of rare earth metals such as lanthanides, especially computer memories. Stirring increase availability WEEE with acid which is produced by Thiobacillus ferrooxidans. It display terbium from alloy. 2) Effluent from our bioreactor has low pH (about 2,5) what is a lethal for E.coli. This is the reason why we add Ca(OH)2 to effluent. It increases our pH to a level which is optimal to E.coli (about 7). Ca(OH)2 is also cheap and it is not increasing cost of the process very much. 3) Effluent goes to packed column bioreactor in which E.coli is immobilized. It gets across whole column. Ions of terbium binds and senses trough periplasmic domain. 4) Column (with no initial effluent) is flushed by NaCl. PmrB can easily denaturate in NaCl solution losing its conformation. Ions of terbium do not precipitate with NaCl aq and do not make insoluble components with it. 5) Ions of terbium are recovered from solution by electrolysis
