Team:WPI-Worcester/Interlab

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Team:WPI-Worcester - 2014.igem.org

 

Team:WPI-Worcester

From 2014.igem.org


The Interlab Study

We found that the construct we built into a chloramphenicol vector (BBa_J23101 + BBa_E024) was drastically more fluourescent than the same construct in a kanamycin vector (BBa_I20260).
We followed the preperation protocol on the official interlab page.
As for our method of measurement, we used 96-well plates and a plate reader that adjusted for OD595. We used non-fluourescent e. coli as a control.

A summary of our results Our Full Results Our Worksheet

Protocols

Digestion Ligation

Parts Used and Sequencing Data

(click to open sequence, double click on the sequence to highlight all nucleotides)

Alternatively, click here to download all of the .ab1 files.
BBa_I20260
NANAACCCTGAAATTAACCTATAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATAATACTAGTAGCGGCCGCTGCAGTCCGGCAAAAAGGCAAGGTGTCACCACCCTGCCCTTTTTCTTAAACCGAAAAAATTAACTTCCGCGGTTATGCAGCTCCTCCTCCTTGACTCGCTTGGCCTCGNCGTCGNTGCGGCNAGCGNATCAGGCTCACCTCAAGCGGTATTCTCTGATTCGGTTCAGTCACCGNATGCCTTGCCAGGTTCACCANNTACCAATCGGATTGAANTCTATCGAGCGTCATAATGATAACATCA
BBa_K823012 + BBa_E0240
ANNTAATCTGTCTTTAACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTATAGCTAGCTCAGTCCTAGGTACAATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTATCTGTTGTTTGTCGGTGACGCTCTCTACTAGAGTCACACTGGCTCACTTCGGTGGGCCTTTCTGCGTTTATATACTAGTAGCCGTCCGCTGCAGTCGCAAAAAGGGCAAGGTGTCACCAACCCTGCCTTTTCTTAATCGAAAAGATACTTCGCGTTATGCAGCTTCTGCTCACTGACTCGCTGCCCTTGGTCGTTCGCTGCGGCGAGCGTATCAGCTACACNNNGNGTAATCCNNNATCCACGAATNGGNNNNCNGNNGAACTGTGACCANGCATCAGGTCGGAACTCTAAAAGGCCGCGTGTTCTT
BBa_J23101 + BBa_E0240
NCNTTATCCTTGAATTACCTATAAAAATAGGCGTATCACGAGGCAGAATTTCAGATAAAAAAAATCCTTAGCTTTCGCTAAGGATGATTTCTGGAATTCGCGGCCGCTTCTAGAGTTTACAGCTAGCTCAGTCCTAGGTATTATGCTAGCTACTAGAGTCACACAGGAAAGTACTAGATGCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAATAATACTAGAGCCAGGCATCAAATAAAACGAAAAGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACTTCGGTGGGCCTTTCTGCGTTTAATATACTAGTAGCGGCGCTGCAGTCGGCAAAAAGGGCAAGTTGTCACCACCCTGCCCTTTTTCTTTAAACGAAAAGAATACTTCGCGGTTATGCAGCTCCTTCGCTCACTGACTCGCTGCGCTCGTCGTCGCTTGCGGCCGAGCGNNTCAGCTCACTCAGCGTATACCGNATCCNNNAATCAGNNACGNGAGACTTGACAAGGCAGCAAGGCCAGAACCGTTAAGCCCGCN