Team:SYSU-China/file/Project/notebook/lab note/mutation

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May: amplify biobricks

June: clone the WT DNAQ ,recA,umuC,umuD from E.coli

July: perform the point-mutation and truncation of the four genes

August: add prefix and suffix to the genes

Add RBS to the genes

September: Construct the polycistron plasmid (DNAQ926+umuD’;umuC+recA730;umuD’+umuC+recA730;DNAQ926+umuD’+umuC+recA730;)

Add promoter to construct the expression vector

Test the mutation rate

October: adapt the testing method and test the mutation rates

Prepare the biobrick for submission

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