The sensor is cultured in the sensor pool of 3mm diameter in the first layer and we call it chamber A.
The Sensor group produces AHL molecules when they are stimulated by radiation. AHL go through the semi-permeable membrane (between the first and second layer) and are transmitted to the amplifier, which is cultured in a three-millimeter-in-diameter pool in the second layer, or say chamber B. afterwards, the amplifier are injected through the one-millimeter-in-diameter chamber C,which is closer to the center in the first layer
.
To make the liquid go through layers, we design a standard channel that drill through the bottom edge of the upper layer and the top edge of the lower layer.
When the liquid in a certain velocity is added at the chamber C, it directly flows into the chamber B through the channel A. Then press on chamber A with an injector to help the AHL molecules go through the semi-permeable membrane. Afterwards, the liquid then go through the long curly channel B on the third layer, and reaches the final five-millimeter-in-diameter detecting pool in the second layer. If the liquid is redundant, it will brim over and flow to the drainage hole in 1mm diameter which is near the edge of the hexagon in the first layer. This structure helps the AHL and amplifier mix sufficiently. Finally amplifier generates fluorescence. Then our device will detect the fluorescence.