Team:NUDT CHINA/Safety

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Safety

Organisms and Parts

Organisms

This year, we used different species of E.coli in our project. Different Genotype provided us different choices when planning our experiment. Based on our project, following 4 species of E.coli was used in our project. All of these species are common and widely used in the field of molecular clone.

StrainSpecies nameGenotypeRish GroupRish Group SourceDisease risk to humans
DH5αEscherichia coliF-,φ80dlacZΔM15,Δ(lacZYA-argF)U169,deoR,recA1,endA1,hsdR17(rk-,mk+),phoA,supE44,λ-,thi-1,gyrA96,relA11PSDSlow infectious dose
Top10Escherichia coliF-, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74, nupG, recA1, araD139, Δ(ara-leu)7697, galE15, galK16, rpsL(StrR), endA1, λ-1PSDSlow infectious dose
BL21(DE3)Escherichia coliF-,ompT, hsdS(rBB-mB-),gal, dcm(DE3)1PSDSlow infectious dose
MC1061/P3Escherichia coliF-, hsdR, (rk-, mk+) araD139, Δ(araABC-leu)7679, galU, galK, ΔlacX74, rpsL, thi, mcrB, {P3: KanR, AmpR,(am) TetR, (am)}1PSDSlow infectious dose

Parts

Our parts are all assembled with materials from the distribution or synthesized based on known sequence. For more detaile, please read the ’’part’’ section of our wiki.

Institutional Rules and Regulations

The NUDT's Commission for Construction of Biological Sciences is responsible for bio-security in our institution. We have discussed the project with the teachers of this commission, and gotten their approval. Three extra teachers led by the deputy chief of DCB(the Department of Chemistry and Biology) were designated to provide us assistance if necessary and monitor us on our implementation on the lab bio-safety protocols at the same time.

Training

All team members were required to attend safety training focused on:

  • General biosafety principles.
  • The proper use of containment equipment (Biological safety (laminar flow) cabinets and delimited working space when required) as well as personal protective equipment (PPE) (White coat and gloves).
  • Risk assessment of experiments involving: Recombinant DNA, ethidium bromide, other bio hazardous materials.
  • Proper Biological waste disposal.

Lab security

toxic chemical reagents EB

Ethidium Bromide (EB) is a very toxic agent. However, because it’s high efficiency and low cost, our lab still use this molecule as nucleic acid staining molecule. To prevent it from hurting us, our lab requires everyone in the electrophoresis Rome to wear a second pair of gloves. Also, Wasted gel were collected and properly disposed.

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