Team:NTNU Trondheim/Project/Modelling

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NTNU Genetically Engineered Machines

Modelling

Flux balance analysis of the impact of increasing CO2-uptake and introducing Glucose Oxidase into Synechocystis sp. PCC6803

To aid in the identification of possible target genes for increasing CO2 uptake in Synechocystis, the most recent model of the metabolic network in Synechocystis was acquired and analyzed with flux balance analysis. The approach initially chosen was simply to fix the CO2 uptake flux above that achieved in the optimal FBA solution, and examining the effect on the growth rate (figure 1).

As can be seen, the growth rate increases from 0 as the CO2 uptake flux is increased from zero to its optimal value (1.19), and decreases after this point. \times

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