5/19/14
Calibrated Nanodrop.
Nanodropped all of our samples. Very low concentration of DNA.
Ordered primers to PCR our g-blocks.

5/22/14:
Struck out plates with pACYC184 with spectinomycin to grow it.
Digested pBR322 with HindIII.
Double digested pBR322 with HindIII/EcoRI.

5/23/14
Ran gel of digests from 5/22/14. Everything as it should be.
Purified the digested plasmids. 
Nanodropped plasmids; results:
	Single digest I: 52.7ng/uL
	Single digest II: 64.5ng/uL
	Double digest I: 26.3ng/uL
	Double digest II: 41.9ng/uL
Phosphatase treated the four digested plasmids. 

5/27/14
Purified phosphatase treated plasmids from 5/23/14. 
Nanodropped the purified plasmids; results:
	Single digest I: 43.4ng/uL
	Single digest II: 53.6ng/uL
	Double digest I: 43.7ng/uL
	Double digest II: 16.5ng/uL

5/28/14