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Team:Heidelberg/AwesomeSheet/Strains
From 2014.igem.org
Lab book
– Strains
| A | B | C | D | E | F | G | H | I | J | K | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | Organism | SuitaBIe for | Expresses | |||||||||
2 | ID | Species | Strain | Description | cloning | expression | T7 Pol | ccdA | growth speed | Genotype | comments | |
3 | 1 | E. coli | TOP10 | Invitrogen cloning strain for chemically competent cells | yes | no | no | no | slow | F- mcrA Δ( mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ( araleu)7697galU galK rpsL (StrR) endA1 nupG | ||
4 | 2 | E. coli | DH10beta | Cloning strain for electrocompetent cells | yes | no | no | no | slow | Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC) | ||
5 | 3 | E. coli | Turbos | NEB Turbos, fast growing cloning strain | yes | no | no | no | medium | F' proA+B+ lacIq ∆lacZM15 / fhuA2 ∆(lac-proAB) glnV galK16 galE15 R(zgb-210::Tn10)TetS endA1 thi-1 ∆(hsdS-mcrB)5 | does a lot of recombination | |
6 | 4 | E. coli | MG1655 | This is the "wild type" K-12 strain which was sequenced, and should be used when PCRing genes from the sequenced genome. It also looks very healthy under the microscope -- a dramatic difference from most of the cloning strains, which appear sick. | no | yes | no | no | fast | F- λ- ilvG- rfb-50 rph-1 | most natural strain | |
7 | 5 | E. coli | One Shot | yes | no | no | yes | slow | F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697galU galK rpsL (StrR) endA1 nupG fhuA::IS2 | |||
8 | 6 | E. coli | BI21(DE3) | no | yes | yes | no | fast | fhuA2 [lon] ompT gal (λ DE3) [dcm] ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5 | |||
9 | 7 | E. coli | Rosetta (DE3) | no | yes | yes | no | ? | F- ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE2 (CamR) | |||
10 | 8 | Bacillus subtilis | 168 | received from Ilka Bischofs | ||||||||
11 | 9 | E. coli | Waste | the most abundant strain | no | no | probably | possibly | extremely fast | WasTE Grb::age ΔtraSh(craP'-dirT) | grows everywhere | |
12 | 10 | |||||||||||
13 | 11 | |||||||||||
14 | 12 | |||||||||||
15 | 13 | |||||||||||
16 | 14 | |||||||||||
17 | 15 | |||||||||||
18 | 16 | |||||||||||
19 | 17 | |||||||||||
20 | 18 | |||||||||||
21 | 19 | |||||||||||
22 | 20 | |||||||||||
23 | 21 | |||||||||||
24 | 22 | |||||||||||
25 | 23 | |||||||||||
26 | 24 | |||||||||||
27 | 25 | |||||||||||
28 | 26 | |||||||||||
