Team:Evry/Notebook/Transformation/23-07-2014

From 2014.igem.org

Revision as of 12:44, 17 October 2014 by NoémieBC (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Picture

Construction of a new plasmid - Choice and amplification of the ORI
PCR with

  • primers 49 and 50 (see primers table in Protocols)
  • Q5 high fidelity enzyme
  • TM=60°C.

    Migration of the PCR products, we have two bands per well.

    Figure
    Figure: Result of ORI amplification in Pseudovibrio denitrificans (Pd), Pseudovribrio ascidiaceicola (Pa) and E.coli DH5a.

    The negative control with E.coli DH5a confirms that there is no amplification. For Pseudovibrio strains, we obtain one band for Pseudovibrio denitrificans and two for Pseudovibrio ascidiaceicola.


    July 23

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transformation/23-07-2014"