Team:Evry/Notebook/Transformation/22-07-2014

From 2014.igem.org

Revision as of 12:38, 17 October 2014 by NoémieBC (Talk | contribs)

Picture

Construction of a new plasmid - Choice and amplification of the ORI
We chose to amplify the genes RepA, RepB and RepC. We find these three genes with the genome browser on the genome of Pseudovibrio FOBEG1.
PCR with

  • primers 49 and 50 (see primers table in Protocols)
  • Q5 high fidelity enzyme
  • TM=55°C.

    Migration of the PCR products, we have no band the amplification didn't work.

    July 18

    • Retrieved from "http://2014.igem.org/Team:Evry/Notebook/Transformation/22-07-2014"