Team:Groningen/Template/MODULE/Notebook/characterisation/week4

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22 - 28 September
 
Fusion of Nis A- terminator and Superfolded GFP-terminator with RBS and purification of the promotor collection (CP1, CP11, CP29, CP30, CP41, CP44)
 
Ligation experiment with PSB2K3 as a backbone and B0034 as 2nd insert
Transformation of overnight ligated sample in E.coli
Size of the construct was determined with the help of colony PCR
Inoculation of the positive clones and plasmid isolation
 
 
 
 
 

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