Team:Paris Saclay/Notebook/July/11
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Contents |
Friday 11th July
Lab work
1 - Fill in the WIKI (Notebook and Protocols)
Juliette & Romain
2 - Transformation of competent cells E.coli
Fabio & Maher
Competent strains used:
- DH5α
- MG1655
- MG1655Z1
- DH5α+ (given by Solenne)
BioBricks used:
- J23100 (Amp R)
- J23106 (Amp R)
- J23114 (Amp R)
+ pET28a (Kan R) (pET28a is a plasmid used to verify that the 4 strains are competent).
For the 3 BB:
Negative control: without plasmid or BB.