Team:Bielefeld-CeBiTec/Results/CO2-fixation

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Module II - Carbon Dioxide (CO2) Fixation

The particular aim of the second module is to realize the carbon dioxide fiaxtion in E. coli. For this approach all items, like the Sedoheptulose-1,7-Bisphpospahtase, the Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) and the mechanism of carbon dioxide fixation a tested separetly in various approaches. For the optimization of the carbon dioxide fixation under aerobic growth conditions we investigate the anerobic microcompartment from Halothiobacillus neapolitnaus, called the carboxysom.


Figure1: Schematic representation of the calvin cylce. The reaction shwon in green can be realized by enzymes that naturally exist in E. coli, while the red one need to be expressed heterologous to fullfill the whole calvin cycle in E. coli.
The different results for all three enzymes are mentioned in the Calvin-cycle section. One important step for the carbon dioxide fixation is the RuBisCO (Ribulose 1,5-bisphosphate carboxylase/oxygenase). We decided to transform DNA sequences into E. coli which encode the carboxysome. Due to its special properties this microcompartiment is very usefull for the carbon dioxide fixation.

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