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PROTOCOLS

PREPARING CELLS FOR TRANSFORMATION (A)

A1V1 Preparing Competent Cells

A2V1 Preparation of Liquid Broth Media

A3V2 Making Agar Plates

A4V1 Measuring OD

 

PREPARING PLASMIDS FOR TRANSFORMATION (B)

B1V2 PCR Protocol

B2V4 DNA Gel Electrophoresis

B3V3 DNA Gel Purification

B4V3 Restriction Digestion

B5V2 Ligation

B6V1 RE-Based Colony Screening

 

TRANSFORMATION AND PROTEIN EXPRESSION (C)

C1V2 Transformation

C2V2 Protein Expression

 

PROTEIN PURIFICATION (D)

D1V1 Column Purification of His-Tagged Proteins

 

PROTEIN ANALYSIS (E)

E1V2 SDS-PAGE

E2V2 Coomassie Staining

E3V6 Western Blotting

 

OTHER (F)

F1V1 pH Meter

F2V2 Cleaning Up

F3V1 Large Autoclave

F4V1 Small Autoclave

 

Project Description

Content

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References

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1. Overall project summary
2. Project Details
3. Materials and Methods
4. The Experiments
5. Results
6. Data analysis
7. Conclusions

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