Team:Paris Bettencourt/Parts

BBa_K1403003 - Limonene synthase

This part consists of a limonene synthase coding sequence downstream of a constitutive promoter from the Anderson's promoter library. We added a promoter and a RBS site to the original part Bba_I742110, making it directly expressed in E.Coli.

The purpose of this plasmid is to produce limonene in E.coli to produce lemon smell for our project of Odor Library.

We used the original biobricks I742110 with promoter J23108 and RBS. It is transformed into Keio collection odorless strain later. Limonene syntheses enzyme converts farnesyl-diphosphate to (+)-limonene, which is a component of lemon scent.

The experiment process was completed by standard cloning. The promoter and RBS was designed as oligos and later aligned, digested and ligated. The finished plasmid is in standard biobricks format.