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Team:Groningen/Template/MODULE/Notebook/secretion/week5
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25 – 29 August
goal: obtaining a construct with the promotors and GFP for promotor analysis in L. lactis.
All the clones containing the constructs with p2+rbs, pLAS+rbs and gfp+Dterm were grown again to be mini-prepped. After this, these products, including ssUSP45DspB ran on a gel showing that p2+rbs and pLAS+rbs had the correct size, but ssUSP45DspB and gfp+Dterm did not have the correct size.
Again, the Gblock of ssUSP45DspB was cloned with the digestion mix, and again rendering negative results.
After a couple of failures with the small amount of gblocks which has been given, PCR was done on 1µl of the digestion mixture. The products which were produced were: ssUSP45 and ssUSP45DspB without His-tag.
