Team:DTU-Denmark/Achievements/Medal fulfillings

Bronze medal requirements

1. Team registration
2. Complete Judging form.
3. Team Wiki.
4. Present a poster and a talk at the iGEM Jamboree.
5. Participate in the Measurement Interlab Study
• As participants in the measurement track we have contributed to the interlab study, by transforming GFP expressed from different promoters into E. coli and measure fluorescence signal with BioLector and FACS. (link to interlab study results)
6. Document at least one new standard BioBrick Part central to your project
• We have submitted two BioBricks essential for our project to the iGEM Registry. This includes the Spinach2 in pSB1C3 backbone with and without a terminator (link to part registration). This BioBrick is new to the registry we have introduced two point mutations to remove an internal SpeI restriction site from the original sequence. The parts have been sequenced and demonstrated to be expressed and bind to the fluorophore DFHBI-1T in E. coli. the parts are registered as BBa_K1330000 and BBa_K1330001.
• Furthermore we intended to streamline the Anderson promoter library by transforming promoters formerly present in plasmid J61002 into the iGEM standard backbone pSB1C3. This includes the promoters J23105, J23110, J23112 and J23116, now registered as K1330002, K1330003, K1330004 and K1330005 respectively. This will be a great advantage for future iGEM teams working with the Anderson promoter Library.

Silver medal requirements

1. Experimentally validate that at least one new BioBrick Part and construction works as expected:
• The Spinach2 fragment has been transformed into E coli. Original Spinach2 and Spinach2 with two point mutations to remove an internal SpeI restriction site, have been demonstrated to fluoresce at equal levels, and can therefore be used for measuring promoter activity. The parts have been demonstrated to fluoresce only after addition of the fluorophore DFHBI-1T. Furthermore the fluorophore was proved not to cause significant fluorescence signal without the spinach. (link to experimental results). We have thereby validated that the novel biobrick works as we expected, and opened up for new ways of measuring absolute promoter strength.
2. Document the characterization of this part in the Registry.
• LINK TO REGISTRY PARTS.
3. Submit this new part to the iGEM Parts Registry
• Our parts have been submitted 2014.09.30

Gold medal requirements