Team:Heidelberg/pages/Part Improvement

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One of the central ideas of iGEM and Synthetic Biology is about modularity, which means standardization and reusing existing parts from the registry. During the course of our project we discovered a very interesting Biobrick by the Milwaukee iGEM Team 2013. It is a Xylanase from Bacillus subtilis; Xylanases degrade Xylan a major wood component. If you want to know more our work with Xylanases and their industrial applications please go to our page on Xylanases.

But as so often with existing things, the part by the Milwaukee Team wasn't a 100% suitable for our needs. That's why we decided to improve the part and its characterization. Although the original enzyme is produced by Bacillus subtilis we were already working in our lab with E. coli. That's why decided to optimize the part for the expression in E. coli.