Team:Carnegie Mellon/Superoxide
From 2014.igem.org
Superoxide Generator Improvement
iGEM Registry of Standard Biological Parts, Part:BBa K1184000
In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods(Source: Carnegie Mellon iGEM 2013)
Methods Used
- Optimization of codons in KillerRed by eliminating rare codon usage.
- Optimization of codons in Supernova, the monomeric version of KillerRed, by eliminating rare codon usage.
- Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.