In 2013, the Carnegie Mellon iGEM team engineered an alternative to antibiotics through the use of phage therapy and the superoxide generator known as KillerRed. The light-activated production of ROS by KillerRed kills bacteria. In 2014, we worked to further characterize the effects of KillerRed using several methods(Source: Carnegie Mellon iGEM 2013)
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Methods Used
Optimization of codons in KillerRed by eliminating rare codon usage.
Optimization of codons in Supernova, the monomeric version of KillerRed, by eliminating rare codon usage.
Photobleaching analysis of KillerRed, Codon Optimized KillerRed, and Codon Optimized Supernova.
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