Team:Groningen/Template/MODULE/projects/parts/secretion

When optimistic bacteria infect start infection a wound they often protect themselves with a biofilm. For an effective fight against these infections the biofilm should be broken down. We have assembled a biobrick of dispersinB fused to an USP45tag (BBa_K1365111). This part is later assembled in the secretion system agains both Staphylococcus aureus and Pseudonomas aeruginosa .

We will fight against P. aeruginosa with an dual action system (BBa_K1365120). We assembled from exciting and novel biobricks a system which is able to produce dispersinB and aiiA (BBa_K1365169). The latter is an quorum quenching enzyme which disrupt communication between P. By doing this we try reduce their activity of both dividing and activating their pathogenic genes (source).

The cell wall of the gram-positive bacteria Staphylococcus aures contains Lipid-2 fatty acids. These are perfect targets for the anti-microbial nisin. We designed and assembled nisin in an a novel biobrick (BBa_K1365100) and in the same brick we included the dispersinB gene. With this, again, dual action system, we want to fight of S. aureus.