Team:Cambridge-JIC/Guide/Constructs/Terminators

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Revision as of 23:15, 16 October 2014 by Salil (Talk | contribs)

Unfortunately, there is a lack of terminators in MP. The only one to find consistent use is the [http://parts.igem.org/Part:BBa_K1484215 nopaline synthase terminator].

This is a terminator found on the endogenous A. tumefaciens T-DNA!

This makes sequencing multi-gene constructs difficult. Slight variations to the sequence can be introduced via PCR, and mutiple repeats of the terminator can be added.

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