Team:SUSTC-Shenzhen/Notebook/CRISPR/Endotoxin-Free-plasmid-purification-for-pX330-mCherry-pX330-mCherry-GAL4

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Team SUSTC-Shenzhen

Notebook

Elements of the endeavor.

Contents


Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4

2014/8/21~2014/8/22


Materials

EndoFree Plasmid Kit (TIANGEN)

Methods

1. Picked up 2 monoclones from each plate (pX330+mCherry12ml&pX330+mCherry+GAL4)
2. Incubate:
Incubate in 18ml ampicillin medium for pX330+mCherry plasmid, and 22ml for pX330+mCherry+GAL4 plasmid. Put in shaker at 37C for 14h [8.12 6:45pm~8.22 8:45 am]
3. Endotoxin Free plasmid purification for pX330+mCherry, pX330+mCherry+GAL4 Performed as the usual protocol provided by TIANGEN plasmid Kit, except that we eluted for three times at the last step with 100ul EB solution.

Results

Concentration（ng/ul） A260/A280 A260/A230
pX330+mCherry 453.7 1.90 2.52
pX330+mCherry+GAL4 644 1.92 2.60

Maintained by the iGEM team SUSTC-Shenzhen.

Licensed under CC BY 4.0.

  • ©2014 SUSTC-Shenzhen

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